Semen samples with minute amounts of sperm, even the single spermatozoon required to fertilize an oocyte cannot be found in the ejaculate. This is primarily because currently, sperm is generally searched for manually under a microscope. The new technology based on dielectrophoresis (DEP) could sort the sperm upon the fact that the head and tail of a sperm cell have unique and independent electrical characteristics. Whereas in the low and high frequency ranges, both the head and tail had negative and positive responses respectively, in the middle range, the tail responded positively, while the head still displayed negative behavior, This would enable sperm manipulation by their tail while simultaneously distancing the head from regions with high electric fields, can lead to an effective and safe automated method for the high throughput isolation of rare sperm.
Safer, automated and label free method for sperm vialbility (head is kept away)
- Low-cost and disposable chips
- Isolate rare sperm cells including immotile (but viable) ones from azoospermic
- Can pre-concentrate sperm in severe oligozoospermic samples
Applications and Opportunities
- Sperm cells sorting (including immotile (but viable) ones from azoospermic)