Long non-coding RNA (lncRNA) molecules have been implicated recently in scaffolded assemblies and are thought to perform various regulatory functions by confining DNA, chromatin regulators, and RNA binding proteins (RBPs), in both time and space. Nevertheless, there is no large-scale methods, resources, or algorithms for molecular scaffold design and construction. Synthertic long coding RNA’s (slncRNA) contain two distinct modules: a DNA binding module, and a protein binding module. The protein binding module is a cassette of binding sites that facilitates binding of multiple RNA-binding proteins, each in several copies. The technology is based on unique triplex-forming-oligo (TFOs) to their triplex target sites (TTS), TTS-TFO, pairs found using high-throughput screens that were developed called Triplex-seq and Triloci-seq. These technologies allow to quantitatively assess the binding affinity of varieties that work both in vivo and in vitro, which enable to determine multiple specific pairs at various buffer conditions. These high-throughput screening approaches allow to determine the rules for synthesizing a high-affinity TTS-TFO pairs, which is the knowledgebase required for more predictably consistent results and elevated specificity of standard gene-editing solutions.
- Scaffold technology that enables integration of high-throughput oligo-library synthesis technology with scaffold synthesis and functional evaluation.
- Enable functional complexity to be assessed and evaluated
- Enable product variability and flexibility per product specifications
Applications and Opportunities
- software for design of functional slncRNAs libaries
- Optical barcoding with slncRNAs
- Genome editing with slncRNAs
- Synthetic Cellulosomes
- Synthetic Natural Products