The Technology
This innovative hyper-expression system significantly boosts protein production in bacteria, offering scalable solutions for biotechnology and alternative protein industries. The system employs synthetic RNA-protein (sRNP) granules to increase protein titers by targeting degradation pathways and enabling compartmentalization of target proteins and/or target mRNA. Utilizing an RNA binding protein (RNP) with an internal disordered region (IDD) together with a synthetic long non-coding RNA (slncRNA) design based on AI-based model, this method achieves remarkable expression enhancements, with production yields increased by up to 50-fold in common bacterial strains like BL21.
The technology also leverages biomolecular condensation to stabilize proteins, preventing degradation and toxicity within host cells by sequestering toxins within a phase-separated nanoparticle. This ensures a broad application range, spanning bacterial and eukaryotic cells. Its universality and adjustable performance through slncRNA valency further highlight its versatility for various gene expression needs, particularly in the clean-tech,food-tech and pharmaceutical sectors.
Advantages
- High Yield: Achieves up to 50x protein production compared to traditional methods.
- Stability: Mitigates protein degradation and toxicity within cells.
- Scalable and Versatile: Applicable across bacteria and mammalian cells.
- Efficient Production: Reduces time and cost for protein synthesis.
- Innovative Approach: Combines sRNP granules with protein containing an IDD to create a biochemical capacitor that stores RNA, proteins and their ligands stably for long durations inside or outside cells allowing for unmatched performance.
Applications
- Biotechnology: Large-scale production of alternative proteins for food-tech industries.
- Pharmaceuticals: Enhanced production of biologics and protein-based therapeutics.
- Research: High-yield protein synthesis for scientific and industrial studies.
- Food Industry: Scalable production of precision fermentation proteins.
- Synthetic Biology: Customizable expression systems for diverse applications.
- Clean-tech: Biochemical capacitor function allows for stable storage of proteins and their ligands in a high density particle making it ideal for extracting rare-earth elements (REE) and other reusable potentially toxic commodities from modern waste streams.
This technology addresses critical bottlenecks in protein production, ensuring sustainability and efficiency for modern biotechnological challenges.
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