Highly-efficient and scalable extracellular vesicles 3D manufacturing platform based on fluidic shear stress

Prof. Shulamit Levenberg | Biomedical Engineering


Pharmaceuticals and Biotechnology

The Technology

Extracellular vesicles (EVs) are nano-sized particles containing proteins and genetic materials secreted by cells. EVs were shown to be relevant as biomarkers as well as to have therapeutic potential within an array of diseases, such as neurological diseases, cardiovascular diseases, and orthopaedic diseases. However, the production of EVs are mainly based on static 2D or 3D culture platforms which do not mimic both blood circulation (perfusion) and mechanical stimulation in the form of shear stress.

The new invention enables flow induced EV (FEVs) production by 3D cultured stem cells stimulated by flow induced shear stress, with 60 fold higher efficiency production is 3D static cultures.


  • Higher production efficiency
  • Extended biological properties for potentially better therapeutic performance
  • Scalable, safe, and cost-effective procedure

Applications and Opportunities

  • Extended biological properties: EVs can be produced under both physiological and pathological flow-induced shear stress conditions for improving their regenerative therapeutic impact.
  • Safer and efficient production approach: large scale production of EVs to meet the demand of EVs research, commercial purpose, and clinical use without the need of expensive and unsafe materials.
arrow Business Development Contacts
Dr. Gal Gur
Director of Business Development, Life Sciences